Canine GDF15 Protein (GDF-DE115)

Express System: E.coli
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Canine GDF15, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Ala197-Val307

Catalog: GDF-DE115

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Product Description	Recombinant Canine GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala197-Val307.
Product Background	Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
Product Category	Recombinant Protein

Target	GDF15
Gene/Protein ID	A0A8C0TNP6
Species	Canine
Exact Sequence	Ala197-Val307
Molecular Weight	The protein has a predicted MW of 13.12 kDa. The protein migrates to 15-20 kDa under reduced (R) condition and 23-26 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity	Immobilized Canine GDF15, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Canine GDF15, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test).
	Immobilized Canine GDF15, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 48.3ng/ml determined by ELISA.
	Canine GDF15 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.