Canine PD-1/PDCD1 Protein (PD1-DM201)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Canine PD-1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human PD-L1, mFc Tag with the EC50 of 30.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.696 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Leu25-Val170

Catalog: PD1-DM201

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Product Description	Recombinant Canine PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Leu25-Val170.
Product Background	Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
Product Category	Recombinant Protein

Target	PD-1/PDCD1
Gene/Protein ID	NP_001301026.1
Species	Canine
Exact Sequence	Leu25-Val170
Molecular Weight	The protein has a predicted MW of 43.00 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Canine PD-1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human PD-L1, mFc Tag with the EC50 of 30.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.696 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Canine PD-1, hFc Tag captured on CM5 Chip via ProtienA can bind Human PD-L1, His Tag with an affinity constant of 0.696 uM as determined in SPR assay (Biacore T200).
	Immobilized Canine PD-1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human PD-L1, mFc Tag with the EC50 of 30.7ng/ml determined by ELISA (QC Test).
	The purity of Canine PD-1 is greater than 95% as determined by SEC-HPLC.
	Canine PD-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.