Canine PDGF R beta/CD140b Protein (PGF-DM1RB)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.203 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gly32-Val532

Catalog: PGF-DM1RB

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Product Description	Recombinant Canine PDGF R beta/CD140b Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly32-Val532.
Product Background	PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites.
Product Category	Recombinant Protein

Target	PDGF R beta/CD140b
Gene/Protein ID	Q6QNF3
Species	Canine
Exact Sequence	Gly32-Val532
Molecular Weight	The protein has a predicted MW of 57.25 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.203 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human PDGFB, His Tag immobilized on CM5 Chip can bind Canine PDGF R beta, His Tag with an affinity constant of 0.203 uM as determined in SPR assay (Biacore T200).
	The purity of Canine PDGF R beta is greater than 95% as determined by SEC-HPLC.
	Canine PDGF R beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.