Canine PDGFA Protein (PDF-DY10A)

  • Express System: Yeast
  • Product Tag: N-His
  • Purity: > 90% as determined by Tris-Bis PAGE
  • Exact Sequence: Ser87-Arg196

Catalog: PDF-DY10A

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Product Description Recombinant Canine PDGFA Protein is expressed from Yeast with His tag at the N-terminus.It contains Ser87-Arg196.

Product Background

 Platelet-derived growth factors (PDGFs) may play an important role in the development of atherosclerosis acting as chemoattractants and mitogens for vascular smooth muscle cells and macrophages. PDGFA, the most known member of PDGF family, plays a crucial role in occurrence and progression of different tumors.

Product Category

 Recombinant Protein

Target

 PDGFA

Gene/Protein ID

 A0A8C0M6T8

Species

 Canine

Exact Sequence

 Ser87-Arg196
Molecular Weight The protein has a predicted MW of 13.62 kDa. Due to glycosylation, the protein migrates to 15-20 kDa and 35-45 kDa based on Tris-Bis PAGE result.

Product Tag

 N-His

Express System

 Yeast
Purity > 90% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCL.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50-for this effect is 5 - 15 ng/mL.
Canine PDGFA on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

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