Name: Human CD117 Protein (CD1-HM117)
Brand: KactusBio
SKU: CD1-HM117-100UG
Price: 264.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.73ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln26-Thr516

Catalog #CD1-HM117

Size

100UG

500UG

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Human CD117 Protein (CD1-HM117) Summary

Product Description	Recombinant Human CD117 Protein is expressed from HEK293 with His tag at the C-Terminus.; It contains Gln26-Thr516.
Product Background	The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human CD117 Protein (CD1-HM117) Specifications/Details

Protein	CD177
Synonyms	SCFR; PBT; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT
Accession	P10721-2
Species	Human
Biotinylated	no
Amino Acid Range	Gln26-Thr516
Molecular Weight	The protein has a predicted MW of 56.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.73ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.73ug/ml determined by ELISA.
	Immobilized Human CD117, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA.
	Human CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-CD117 Ab., hFc Tag on ProA-Biosensor can bind Human CD117, His Tag with an affinity constant of 6.08 nM as determined in BLI assay (Gator® Prime).
	The purity of Human CD117 is greater than 95% as determined by SEC-HPLC.