Human CD8 alpha/CD8A Protein (CD8-HM18A)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human CD8 alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 29.4ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser22-Asp182

Catalog: CD8-HM18A

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Product Description	Recombinant Human CD8 alpha/CD8A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser22-Asp182.
Product Background	CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I, favoring lipid raft partitioning of TCR, and inducing intracellular signaling. CD8 alpha is also found on dendritic cells and rat macrophages, but whether CD8 alpha enhances responses of a partner receptor, like TCR, to activate these cells is not known.
Product Category	Recombinant Protein

Target	CD8 alpha/CD8A
Gene/Protein ID	P01732-1
Species	Human
Exact Sequence	Ser22-Asp182
Molecular Weight	The protein has a predicted MW of 18.70 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD8 alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 29.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD8 alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 29.4ng/ml determined by ELISA.
	Human CD8 alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.