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Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM201) Summary
Product Description |
Recombinant Cynomolgus CD3E/CD3 epsilon Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln22-Asp117. |
Product Background |
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM201) Specifications/Details
Protein |
CD3E/CD3 epsilon |
Synonyms |
CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gln22-Asp117 |
Molecular Weight |
The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus CD3E, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD3E Antibody, hFc Tag with the EC50 of 14.9ng/ml determined by ELISA (QC Test). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Cynomolgus CD3E, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD3E Antibody, hFc Tag with the EC50 of 14.9ng/ml determined by ELISA (QC Test). |
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Cynomolgus CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus CD3E is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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