Human CD40 Ligand/TNFSF5 Protein (CDL-HE14L)

Express System: E.coli
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human CD40 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.7ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln114-Leu261

Catalog: CDL-HE14L

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Product Description	Recombinant Human CD40 Ligand/TNFSF5 Protein is expressed from E.coli with His tag at the C-terminus.It contains Gln114-Leu261.
Product Background	CD40 ligand or CD40L, also called CD154,- is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Product Category	Recombinant Protein

Target	CD40 Ligand/TNFSF5
Gene/Protein ID	P29965
Species	Human
Exact Sequence	Gln114-Leu261
Molecular Weight	The protein has a predicted MW of 17.15 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Human CD40 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.7ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 10% glycerol (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human CD40 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.7ug/ml determined by ELISA.
	Human CD40 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.