Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A1 (KVLEYVIKV) Monomer Protein (MHC-HM103)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: The affinity constant of 58.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and KVLEYVIKV peptide

Catalog: MHC-HM103

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Product Description Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A1 (KVLEYVIKV) Monomer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and KVLEYVIKV peptide.

Product Background

 MAGE-A1 belongs to the chromosome X-clustered genes of cancer-testis antigen family and is normally expressed in the human germ line but is also overexpressed in various tumors.

Product Category

 Recombinant Protein

Target

 HLA-A*02:01 (mα3) &mB2M&MAGE-A1 (KVLEYVIKV) Monomer
MHC Form Monomer
Allele HLA-A*02:01

Gene/Protein ID

 A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&&P01887(Mouse B2M)&KVLEYVIKV

Species

 Human&Mouse

Exact Sequence

 Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and KVLEYVIKV peptide
Molecular Weight The protein has a predicted MW of 48.00 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.

Activity

 The affinity constant of 58.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Chimeric HLA-A*02:01(mα3)&mB2M&MAGE-A1 (KVLEYVIKV) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) TCR with an affinity constant of 58.68 nM as determined in SPR assay (Biacore T200).
The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A1 (KVLEYVIKV) Monomer is greater than 95% as determined by SEC-HPLC.
Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A1 (KVLEYVIKV) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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