Cynomolgus APOH Protein (APH-CM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus APOH, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 89.8ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gly20-Cys345

Catalog: APH-CM101

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Product Description	Recombinant Cynomolgus APOH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Cys345.
Product Background	Apolipoprotein (apo)H (also known as beta 2 glycoprotein-I) is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. APOH displays a genetically determined structural polymorphism: three alleles (APOH1, APOH2, APOH3) at a single locus on chromosome 17 code for different isoforms, and population studies have shown that APOH2 is the most frequent allele.
Product Category	Recombinant Protein

Target	APOH
Gene/Protein ID	XP_045230479.1
Species	Cynomolgus
Exact Sequence	Gly20-Cys345
Molecular Weight	The protein has a predicted MW of 37.39 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus APOH, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 89.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus APOH, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 89.8ng/ml determined by ELISA.
	The purity of Cynomolgus APOH is greater than 95% as determined by SEC-HPLC.
	Cynomolgus APOH on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.