Cynomolgus BTLA Protein (BTL-CM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus BTLA, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Lys31-Pro152

Catalog: BTL-CM101

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Product Description	Recombinant Cynomolgus BTLA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys31-Pro152.
Product Background	B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.
Product Category	Recombinant Protein

Target	BTLA
Gene/Protein ID	A0A2K5W7M7
Species	Cynomolgus
Exact Sequence	Lys31-Pro152
Molecular Weight	The protein has a predicted MW of 15.04 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus BTLA, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus BTLA, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
	The purity of Cynomolgus BTLA is greater than 95% as determined by SEC-HPLC.
	Cynomolgus BTLA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.