Cynomolgus CA9/Carbonic Anhydrase IX Protein (CA9-CM101)
Catalog: CA9-CM101
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| Product Description | Recombinant Cynomolgus CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln38-Asp402. |
Product Background |
CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways. |
Product Category |
Recombinant Protein |
Target |
CA9/Carbonic Anhydrase IX |
Gene/Protein ID |
A0A2K5VQG9 |
Species |
Cynomolgus |
Exact Sequence |
Gln38-Asp402 |
| Molecular Weight | The protein has a predicted MW of 41.03 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Cynomolgus CA9, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Cynomolgus CA9, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. |
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Measured by its esterase activity. The specific activity is > 55 pmol/min/µg. |
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The purity of Cynomolgus CA9 is greater than 95% as determined by SEC-HPLC. |
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Cynomolgus CA9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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