Cynomolgus CD27 Ligand/CD70 Protein (CD7-CM170)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;
  • Activity: Immobilized Cynomolgus CD27 Ligand, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD27, hFc Tag with the EC50 of 41.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln39-Pro194

Catalog: CD7-CM170

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Product Description Recombinant Cynomolgus CD27 Ligand/CD70 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln39-Pro194.

Product Background

 CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.- CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

Product Category

 Recombinant Protein

Target

 CD27 Ligand/CD70
Synonyms CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG;-Ki-24 antigen; TNFSF7G

Gene/Protein ID

 G7PYU6-1

Species

 Cynomolgus

Exact Sequence

 Gln39-Pro194
Molecular Weight The protein has a predicted MW of 18.4 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Cynomolgus CD27 Ligand, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD27, hFc Tag with the EC50 of 41.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

 N-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD27 Ligand, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD27, hFc Tag with the EC50 of 41.3ng/ml determined by ELISA.
Cynomolgus CD27 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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