Cynomolgus CD55 Protein (CD5-CM105)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD55, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 68.6ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp36-Ser350

Catalog: CD5-CM105

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Product Description Recombinant Cynomolgus CD55 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp36-Ser350.

Product Background

Decay Accelerating Factor (or CD55) is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection.

Product Category

Recombinant Protein

Target

CD55
Synonyms DAF-GPI; CD55; Cd55a; Daf; Daf1; DAF; CR; CROM; TC

Gene/Protein ID

A0A2K5X1Z6

Species

Cynomolgus

Exact Sequence

Asp36-Ser350
Molecular Weight The protein has a predicted MW of 35.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus CD55, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 68.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD55, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 68.6ng/ml determined by ELISA.
The purity of Cynomolgus CD55 is greater than 95% as determined by SEC-HPLC.
Cynomolgus CD55 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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