Cynomolgus GITR/TNFRSF18 Protein (GTR-CM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Cynomolgus GITR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.21 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln20-Pro156

Catalog: GTR-CM101

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Product Description	Recombinant Cynomolgus GITR/TNFRSF18 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln20-Pro156.
Product Background	GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
Product Category	Recombinant Protein

Target	GITR/TNFRSF18
Gene/Protein ID	XP_005545180.2
Species	Cynomolgus
Exact Sequence	Gln20-Pro156
Molecular Weight	The protein has a predicted MW of 15.87 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus GITR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.21 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus GITR Ligand, His Tag immobilized on CM5 Chip can bind Cynomolgus GITR, His Tag with an affinity constant of 0.21 uM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus GITR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA (QC Test).
	Cynomolgus GITR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.