Cynomolgus GM-CSF R alpha Protein (GSF-CM1RA)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus GM-CSF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Leu20-Ser317

Catalog: GSF-CM1RA

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Product Description	Recombinant Cynomolgus GM-CSF R alpha Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu20-Ser317.
Product Background	In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.
Product Category	Recombinant Protein

Target	GM-CSF R alpha
Gene/Protein ID	A0A2K5UC70
Species	Cynomolgus
Exact Sequence	Leu20-Ser317
Molecular Weight	The protein has a predicted MW of 35.47 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus GM-CSF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus GM-CSF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
	The purity of Cynomolgus GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.
	Cynomolgus GM-CSF R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.