Cynomolgus IFN gamma/IFNG Protein (IFN-CM10G)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus IFN gamma, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human IFNGR1, hFc Tag with the EC50 of 39.4ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln24-Gln165

Catalog: IFN-CM10G

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Cynomolgus IFN gamma/IFNG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln24-Gln165.
Product Background	IFN-gamma and one of its receptor subunits, IFNGR1, are translocated to the nucleus, together with STAT1alpha as one macromolecular complex, via the classical importin-dependent pathway.Put IFN-gamma and its receptor subunit, IFNGR1, in direct contact with the promoter region of IFN-gamma-activated genes with associated increased activity, thus suggesting a transcriptional/cotranscriptional role for IFN-gamma/IFNGR1 as well as a possible role in determining the specificity of IFN-gamma action.
Product Category	Recombinant Protein

Target	IFN gamma/IFNG
Gene/Protein ID	P63309
Species	Cynomolgus
Exact Sequence	Gln24-Gln165
Molecular Weight	The protein has a predicted MW of 17.72 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus IFN gamma, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human IFNGR1, hFc Tag with the EC50 of 39.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus IFN gamma, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human IFNGR1, hFc Tag with the EC50 of 39.4ng/ml determined by ELISA.
	The purity of Cynomolgus IFN gamma is greater than 95% as determined by SEC-HPLC.
	Cynomolgus IFN gamma on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.