Cynomolgus IL-1 Beta/IL-1F2 Protein (IL1-CE10B)
Catalog: IL1-CE10B
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| Product Description | Recombinant Cynomolgus IL-1 Beta/IL-1F2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Ala117-Ser268. |
Product Background |
Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells. |
Product Category |
Recombinant Protein |
Target |
IL-1 Beta/IL-1F2 |
Gene/Protein ID |
A0A2K5VDC7 |
Species |
Cynomolgus |
Exact Sequence |
Ala117-Ser268 |
| Molecular Weight | The protein has a predicted MW of 18.33 kDa same as Tris-Bis PAGE result. |
Activity |
The affinity constant of 1.40 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
E.coli |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS,100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human IL-1 RII, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-1 Beta, His Tag with an affinity constant of 1.40 nM as determined in SPR assay (Biacore T200). |
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The purity of Cynomolgus IL-1 Beta is greater than 95% as determined by SEC-HPLC. |
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Cynomolgus IL-1 Beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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