Cynomolgus IL-13 Protein (IL1-CM113)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 21.16 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ser19-Asn132

Catalog: IL1-CM113

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Cynomolgus IL-13 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ser19-Asn132.
Product Background	Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages.
Product Category	Recombinant Protein

Target	IL-13
Gene/Protein ID	Q0PW92
Species	Cynomolgus
Exact Sequence	Ser19-Asn132
Molecular Weight	The protein has a predicted MW of 13.59 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 21.16 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus IL-13Ra1, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-13, His Tag with an affinity constant of 21.16 nM as determined in SPR assay (Biacore T200).
	The purity of Cynomolgus IL-13 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus IL-13 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.