Cynomolgus IL-13Ra1 Protein (ILR-CM1R1)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 21.16 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala25-Thr341

Catalog: ILR-CM1R1

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Product Description	Recombinant Cynomolgus IL-13Ra1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala25-Thr341.
Product Background	Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
Product Category	Recombinant Protein

Target	IL-13Ra1
Gene/Protein ID	XP_005594500.1
Species	Cynomolgus
Exact Sequence	Ala25-Thr341
Molecular Weight	The protein has a predicted MW of 37.59 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 21.16 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus IL-13Ra1, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-13, His Tag with an affinity constant of 21.16 nM as determined in SPR assay (Biacore T200).
	The purity of Cynomolgus IL-13Ra1 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus IL-13Ra1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.