Cynomolgus IL-18 R1/CD218a Protein (IL8-CM1R1)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 2.02 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Cys22-Gly329

Catalog: IL8-CM1R1

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Product Description	Recombinant Cynomolgus IL-18 R1/CD218a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys22-Gly329.
Product Background	Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
Product Category	Recombinant Protein

Target	IL-18 R1/CD218a
Gene/Protein ID	A0A2K5V3J9
Species	Cynomolgus
Exact Sequence	Cys22-Gly329
Molecular Weight	The protein has a predicted MW of 36.45 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 2.02 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus IL-18 R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.02 nM as determined in SPR assay (Biacore T200).
	The purity of Cynomolgus IL-18 R1 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus IL-18 R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.