Cynomolgus IL-5 Protein (IL5-CM101)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus IL-5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 21.0ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ile20-Ser134

Catalog: IL5-CM101

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Product Description	Recombinant Cynomolgus IL-5 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ile20-Ser134.
Product Background	IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).
Product Category	Recombinant Protein

Target	IL-5
Gene/Protein ID	A0A2K5U1E7
Species	Cynomolgus
Exact Sequence	Ile20-Ser134
Molecular Weight	The protein has a predicted MW of 14.25 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus IL-5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 21.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus IL-5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 21.0ng/ml determined by ELISA.
	The purity of Cynomolgus IL-5 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus IL-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.