Cynomolgus IL-6 Protein (IL6-CM001)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus IL-6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala28-Met212

Catalog: IL6-CM001

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Product Description Recombinant Cynomolgus IL-6 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala28-Met212.

Product Background

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.

Product Category

Recombinant Protein

Target

IL-6

Gene/Protein ID

P79341

Species

Cynomolgus

Exact Sequence

Ala28-Met212
Molecular Weight The protein has a predicted MW of 22.06 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus IL-6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus IL-6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA.
The purity of Cynomolgus IL-6 is greater than 95% as determined by SEC-HPLC.
Cynomolgus IL-6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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