Cynomolgus MMP-8 Protein (MMP-CM108)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus MMP-8, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA (QC tested). See testing image for detail.
Exact Sequence: Phe21-Ser467

Catalog: MMP-CM108

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Product Description	Recombinant Cynomolgus MMP-8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe21-Ser467.
Product Background	Alteration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) expression has been studied for various cardiac diseases, including dilated cardiomyopathy (DCM), with the significance of surrogate markers of extracellular matrix (ECM) remodeling. MMP-8 was identified only in myocardiocytes, while MMP-9 and TIMP-2 were present in both myocardiocytes and stroma, but with different intensity. The increasing intensity of MMP-8 and TIMP-2 immunoreactions was significantly associated with low HCS.
Product Category	Recombinant Protein

Target	MMP-8
Gene/Protein ID	XP_005579523.2
Species	Cynomolgus
Exact Sequence	Phe21-Ser467
Molecular Weight	The protein has a predicted MW of 52.27 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus MMP-8, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA (QC tested). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus MMP-8, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA (QC tested).
	Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2, The specific activity is >200 pmol/min/ug (QC tested).
	The purity of Cynomolgus MMP-8 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus MMP-8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.