Cynomolgus NKG2A&CD94 Protein (NKC-CM194)

Express System: HEK293
Product Tag: C-His, C-Flag
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Cynomolgus NKG2A&CD94, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 8.7g/ml determined by ELISA. See testing image for detail.
Exact Sequence: Pro94-Leu233(NKG2A) & Lys32-Ile179(CD94)

Catalog: NKC-CM194

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NKC-CM194 Product Description	Recombinant Cynomolgus NKG2A&CD94 Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus.It contains Pro94-Leu233(NKG2A)&Lys32-Ile179(CD94).
Product Background	The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
Product Category	Recombinant Protein

Target	NKG2A&CD94
Synonyms	CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
Gene/Protein ID	Q68VD2(NKG2A)&Q68VD4(CD94)
Species	Cynomolgus
Exact Sequence	Pro94-Leu233(NKG2A) & Lys32-Ile179(CD94)
Molecular Weight	The protein has a predicted MW of 17.01 kDa(NKG2A)&18.27 kDa(CD94). Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus NKG2A&CD94, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 8.7g/ml determined by ELISA. See testing image for detail.
Product Tag	C-His, C-Flag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus NKG2A&CD94, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 8.7g/ml determined by ELISA.
	Cynomolgus NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.