Cynomolgus NKG2D/CD314 Protein (NKG-CM12D)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus NKG2D, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ile73-Val216

Catalog: NKG-CM12D

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Product Description	Recombinant Cynomolgus NKG2D/CD314 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ile73-Val216.
Product Background	NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
Product Category	Recombinant Protein

Target	NKG2D/CD314
Gene/Protein ID	P61252
Species	Cynomolgus
Exact Sequence	Ile73-Val216
Molecular Weight	The protein has a predicted MW of 17.48 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus NKG2D, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus NKG2D, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA.
	The purity of Cynomolgus NKG2D is greater than 95% as determined by SEC-HPLC.
	Cynomolgus NKG2D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.