Cynomolgus PCSK9 Protein (PCS-CM190)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus PCSK9, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve Anti-PCSK9 Antibody, hFc Tag with the EC50 of 81.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.27 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu151-Gln811

Catalog: PCS-CM190

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Product Description Recombinant Cynomolgus PCSK9 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu151-Gln811.

Product Background

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is an enzyme encoded by the PCSK9 gene in humans on chromosome 1.The first two PCSK9 inhibitors, alirocumab and evolocumab, were approved as once every two week injections, by the U.S. Food and Drug Administration in 2015 for lowering LDL-particle concentrations when statins and other drugs were not sufficiently effective or poorly tolerated.

Product Category

Recombinant Protein

Target

PCSK9

Gene/Protein ID

A0A2K5TZC4

Species

Cynomolgus

Exact Sequence

Glu151-Gln811
Molecular Weight Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-70 kDa (pro-form) and 15-20 kDa (mature form) based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus PCSK9, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve Anti-PCSK9 Antibody, hFc Tag with the EC50 of 81.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.27 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus LDLR, His Tag immobilized on CM5 Chip can bind Cynomolgus PCSK9, His Tag with an affinity constant of 0.27 nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus PCSK9, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve Anti-PCSK9 Antibody, hFc Tag with the EC50 of 81.5ng/ml determined by ELISA (QC Test).
The purity of Cynomolgus PCSK9 is greater than 95% as determined by SEC-HPLC.
Cynomolgus PCSK9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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