Cynomolgus PDGF R beta/CD140b Protein (PGF-CM1RB)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus PDGFR beta, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PDGFRB Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu33-Lys531

Catalog: PGF-CM1RB

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Product Description Recombinant Cynomolgus PDGF R beta/CD140b Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu33-Lys531.

Product Background

 Platelet-derived growth factor receptor (PDGFR) signaling is involved in proliferation and survival in a wide array of cell types.PDGFR-β signalling, via TGF-β signalling, may be crucial for restoration of BBB integrity after cerebral ischemia and therefore represents a novel potential therapeutic target.

Product Category

 Recombinant Protein

Target

 PDGF R beta/CD140b

Gene/Protein ID

 A0A2K5TUS0

Species

 Cynomolgus

Exact Sequence

 Leu33-Lys531
Molecular Weight The protein has a predicted MW of 57.49 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Cynomolgus PDGFR beta, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PDGFRB Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus PDGFR beta, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PDGFRB Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
The purity of Cynomolgus PDGF R beta is greater than 95% as determined by SEC-HPLC.
Cynomolgus PDGF R beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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