Cynomolgus/Rhesus macaque DKK1-Protein (DKK-CM301)
Catalog: DKK-CM301
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| Product Description | Recombinant Cynomolgus/Rhesus macaque DKK1-Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Thr32-His266. |
Product Background |
Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. |
Product Category |
Recombinant Protein |
Target |
DKK1 |
Gene/Protein ID |
XP_005565907.1/NP_001247454.1 |
Species |
Cynomolgus/Rhesus macaque |
Exact Sequence |
Thr32-His266 |
| Molecular Weight | The protein has a predicted MW of 51.42 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Cynomolgus/Rhesus macaque DKK1, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-mFc |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Cynomolgus/Rhesus macaque DKK1, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. |
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The purity of Cynomolgus/Rhesus macaque DKK1 is greater than 95% as determined by SEC-HPLC. |
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Cynomolgus/Rhesus macaque DKK1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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