Cynomolgus SOST/Sclerostin Protein (SOT-CM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Cynomolgus SOST, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln69-Tyr258

Catalog: SOT-CM101

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Product Description	Recombinant Cynomolgus SOST/Sclerostin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln69-Tyr258.
Product Background	SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Product Category	Recombinant Protein

Target	SOST/Sclerostin
Gene/Protein ID	XP_005584428.2
Species	Cynomolgus
Exact Sequence	Gln69-Tyr258
Molecular Weight	The protein has a predicted MW of 22.58 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus SOST, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus SOST, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.
	Cynomolgus SOST on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.