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Human Ephrin-B2/EFNB2 Protein (ENB-HM201) Summary
Product Description |
Recombinant Human Ephrin-B2/EFNB2 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ile28-Ala229. |
Product Background |
Ephrin-B2 controls platelet-derived growth factor receptor β (PDGFRβ) distribution in the VSMC plasma membrane, endocytosis, and signaling in a fashion that is highly distinct from its role in the endothelium. Ephrin-B2 is an important regulator of PDGFRβ endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor in mural cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Ephrin-B2/EFNB2 Protein (ENB-HM201) Specifications/Details
Protein |
Ephrin-B2/EFNB2 |
Synonyms |
Ephrin B2; EPLG5; HTKL; LERK5; EFNB2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ile28-Ala229 |
Molecular Weight |
The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human EPHB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 82.97 pM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human Ephrin-B2, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200). |
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Immobilized Human EPHB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). |
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Human Ephrin B2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Ephrin B2 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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