Regular price
$371.80
Order by 4pm ET for delivery on:
Cynomolgus FAP Protein (active dimer) (FAP-CM101) Summary
Product Description |
Recombinant Cynomolgus FAP Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu26-Asp760. |
Product Background |
Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets, ADC targets |
Cynomolgus FAP Protein (active dimer) (FAP-CM101) Specifications/Details
Protein |
FAP |
Synonyms |
FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Leu26-Asp760 |
Molecular Weight |
The protein has a predicted MW of 86.2 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus FAP at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). See testing image for detail.Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >7000-pmol/min/µg (QC Test). |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Cynomolgus FAP at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). |
|
|
Cynomolgus FAP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Cynomolgus FAP is greater than 95% and the molecular weight of this protein is around 175-200 kDa as determined by SEC-MALS. |
|
|
The purity of Cynomolgus FAP is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
