Ferret TNF alpha-Protein (TNF-FE00A)

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Ferret TNF-alpha, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 14.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Val77-Leu233

Catalog: TNF-FE00A

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Ferret TNF alpha-Protein is expressed from E.coli without tag.It contains Val77-Leu233.
Product Background	Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Product Category	Recombinant Protein

Target	TNF alpha
Gene/Protein ID	A3FBF1
Species	Ferret
Exact Sequence	Val77-Leu233
Molecular Weight	The protein has a predicted MW of 17.21 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Ferret TNF-alpha, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 14.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Ferret TNF-alpha, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 14.5ng/ml determined by ELISA.
	The purity of Ferret TNF alpha is greater than 95% as determined by SEC-HPLC.
	Ferret TNF alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.