• Express System: E.coli
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 100.7ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Phe16-Asp155
Catalog #FGF-HE40AB
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human FGF1 Protein (FGF-HE40AB) Summary

Product Description

 Recombinant Biotinylated Human FGF1 Protein is expressed from E.coli with His tag and Avi tag at the N-terminus.It contains Phe16-Asp155.

Product Background

 Fibroblast Growth Factor 1 (Fgf1), also known as acidic FGF (aFGF), is involved in the regulation of various biological processes, ranging from development to disease pathogenesis. It is a single chain polypeptide and is highly expressed in adult brain and kidney tissues. Its expression has been shown to be directed by multiple tissue-specific promoters, which generate transcripts of varying lengths.

Biotinylated Human FGF1 Protein (FGF-HE40AB) Specifications/Details

Protein
 FGF1
Synonyms
 FGF-1; aFGF; ECGF; HBGF-1; FGFA;FGF1; HBGF1;Fibroblast growth factor-1; Fibroblast growth factor 1
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Phe16-Asp155
Molecular Weight
 The protein has a predicted MW of 18.74 kDa. Due to glycosylation, the protein migrates to 20-24 kDa based on Tris-Bis PAGE result.
Activity
 Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 100.7ng/ml determined by ELISA (QC Test).
Product Tag
 N-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 100.7ng/ml determined by ELISA (QC Test). Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 100.7ng/ml determined by ELISA (QC Test).
Biotinylated Human FGF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human FGF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...