FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer Protein (MHC-HM44RTF)

  • Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized Anti-HLA class I (W6/32) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer, His Tag with the EC50 of 2.55ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*03:01) & Ile21-Met119(B2M)

Catalog: MHC-HM44RTF

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Product Description Recombinant FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*03:01) and Ile21-Met119 (B2M).

Product Background

 Peptide Ready HLA-A*03:01&B2M Tetramer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*03:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Product Category

 Recombinant Protein

Target

 Peptide Ready HLA-A*03:01&B2M
MHC Form Tetramer
Allele HLA-A*03:01

Gene/Protein ID

 NP_002107.3(HLA-A*03:01)&P61769(B2M)

Species

 Human

Exact Sequence

 Gly25-Thr305(HLA-A*03:01) & Ile21-Met119(B2M)
Molecular Weight The protein has a predicted MW of 301.2 kDa.

Activity

 Immobilized Anti-HLA class I (W6/32) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer, His Tag with the EC50 of 2.55ug/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His

Fluorophore

 FITC

Express System

 HEK293
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-HLA class I (W6/32) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer, His Tag with the EC50 of 2.55ug/ml determined by ELISA.

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