FITC-Labeled Human MSLN/Mesothelin Protein (MSL-HM280F)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Exact Sequence: Glu296-Gly580

Catalog: MSL-HM280F

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Product Description Recombinant FITC-Labeled Human MSLN/Mesothelin Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu296-Gly580.

Product Background

 Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

Product Category

 Recombinant Protein

Target

 MSLN/Mesothelin
Synonyms MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR

Gene/Protein ID

 Q13421-2

Species

 Human

Exact Sequence

 Glu296-Gly580
Molecular Weight The protein has a predicted MW of 59 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.

Product Tag

 C-hFc

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FACS Analysis of Anti-MSLN CAR Expression. 293T cells were transfected with anti-MSLN-scFv and Fc tag. Cells were stained with 5ug/ml FITC-Labeled Human MSLN (296-580) , Fc Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control.
FITC-Labeled Human MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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