Hamster FGF21-Protein (FGF-HM12T)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Activity: The affinity constant of 264.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Arg29-Ser209

Catalog: FGF-HM12T

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Product Description	Recombinant Hamster FGF21-Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg29-Ser209.
Product Background	Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
Product Category	Recombinant Protein

Target	FGF21
Gene/Protein ID	XP_005084765.1
Species	Hamster
Exact Sequence	Arg29-Ser209
Molecular Weight	The protein has a predicted MW of 20.80 kDa. Due to glycosylation, the protein migrates to 21-30 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 264.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Hamster FGF21, His Tag immobilized on CM5 Chip can bind Human Beta Klotho, His Tag with an affinity constant of 264.50 nM as determined in SPR assay (Biacore T200).
	The purity of Hamster FGF21 is greater than 90% as determined by SEC-HPLC.
	Hamster FGF21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.