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Canine HE4 Protein (HE4-DM204) Summary
Product Description |
Recombinant Canine HE4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly28-Phe124. |
Product Background |
The ovarian cancer biomarker HE4 is known to promote proliferation, metastasis, chemoresistance, and suppression of cytotoxic lymphocytes. HE4 has the ability to affect signaling events and gene expression in multiple cell types of the tumor microenvironment, which could contribute to angiogenesis and altered immunogenic responses in ovarian cancer. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Canine HE4 Protein (HE4-DM204) Specifications/Details
Protein |
HE4 |
Synonyms |
Epididymal secretory protein E4; CE4; WFDC2; WAP5; EDDM4 |
Accession |
|
Species |
Canine |
Biotinylated |
no |
Amino Acid Range |
Gly28-Phe124 |
Molecular Weight |
The protein has a predicted MW of 37.06 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Canine HE4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Canine HE4 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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