Human Activin A Protein (ACV-HM001)

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Activin RIIB, His Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Gly311-Ser426

Catalog: ACV-HM001

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Product Description	Recombinant Human Activin A Protein is expressed from HEK293 without tag.It contains Gly311-Ser426.
Product Background	Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.
Product Category	Recombinant Protein

Target	Activin A
Gene/Protein ID	P08476
Species	Human
Exact Sequence	Gly311-Ser426
Molecular Weight	The protein has a predicted MW of 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-25 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity	Immobilized Human Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Activin RIIB, His Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	No Tag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 0.1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 4mM HCI. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCI.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Activin RIIB, His Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test).
	Immobilized Human Activin A, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Activin RIIA, His Tag with the EC50 of 0.23ug/ml determined by ELISA.
	Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL (QC Test).
	Human Activin A on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.