Human APCDD1 Protein (APD-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human APCDD1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-APCDD1 Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Leu27-His492

Catalog: APD-HM101

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Product Description	Recombinant Human APCDD1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu27-His492.
Product Background	Adenomatosis polyposis coli downregulated 1 (APCDD1), a negative regulator of Wnt signaling, was examined to understand detailed mechanisms underlying Wnt signaling tooth development. In situ hybridization showed that Apcdd1 was expressed in the condensed mesenchyme at the bud stage, and in the inner enamel epithelium (IEE), including enamel knot (EK) at the cap stage. APCDD1 modulates the gene expression of Wnt- and EK-related signaling molecules at the cap stage of tooth development, and is involved in tooth cusp patterning by modulating the epithelial rearrangement in the IEE.
Product Category	Recombinant Protein

Target	APCDD1
Synonyms	Apcdd1; Drapc1; B7323; FP7019; HHS; HTS; HYPT1
Gene/Protein ID	Q8J025
Species	Human
Exact Sequence	Leu27-His492
Molecular Weight	The protein has a predicted MW of 54.4 kDa. Due to glycosylation, the protein migrates to 58-68 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human APCDD1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-APCDD1 Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human APCDD1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-APCDD1 Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA.
	Human APCDD1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.