Human APOE2/Apolipoprotein E Protein (APO-HM1E2)
Catalog: APO-HM1E2
Order now get it on
| Product Description | Recombinant Human APOE2/Apolipoprotein E Protein is expressed from HEK293 with His tag at the C-terminus.It contains Lys19-His317(R176C). |
Product Background |
Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD). |
Product Category |
Recombinant Protein |
Target |
APOE2/Apolipoprotein E |
Gene/Protein ID |
P02649-1 |
Species |
Human |
Exact Sequence |
Lys19-His317(R176C) |
| Molecular Weight | The protein has a predicted MW of 35.28 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 27.11 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE2, His Tag with an affinity constant of 27.11 nM as determined in SPR assay (Biacore T200). |
|
|
The purity of Human APOE2 is greater than 95% as determined by SEC-HPLC. |
|
|
Human APOE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
You may also be interested in
View more associated products