Human-AREG-Protein (AEG-HM201)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human AREG, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser101-Lys187

Catalog: AEG-HM201

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Product Description	Recombinant Human-AREG-Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ser101-Lys187.
Product Background	Amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and is expressed in a plethora of cancers. Tumour growth and metastasis were decreased by AREG silencing in an orthotopic model of pancreatic cancer. AREG may play a critical role in cell migration, invasion, and EMT by activating the EGFR/ERK/NF‑κB signalling pathway in pancreatic cancer cells.
Product Category	Recombinant Protein

Target	AREG
Synonyms	Amphiregulin; AR; SDGF; AREG; AREGB; CRDGF; MGC13647
Gene/Protein ID	P15514
Species	Human
Exact Sequence	Ser101-Lys187
Molecular Weight	The protein has a predicted MW of 37.3 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human AREG, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human AREG, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA.
	The purity of Human-AREG is greater than 95% as determined by SEC-HPLC.
	Human-AREG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.