Human B7-2/CD86 Protein (B72-HM286)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CTLA-4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-2, hFc Tag with the EC50 of 125.7ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Leu26-Pro247

Catalog: B72-HM286

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Product Description	Recombinant Human B7-2/CD86 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu26-Pro247.
Product Background	B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
Product Category	Recombinant Protein

Target	B7-2/CD86
Synonyms	CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2
Gene/Protein ID	P42081-1
Species	Human
Exact Sequence	Leu26-Pro247
Molecular Weight	The protein has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CTLA-4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-2, hFc Tag with the EC50 of 125.7ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CTLA-4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-2, hFc Tag with the EC50 of 125.7ng/ml determined by ELISA.
	The purity of Human B7-2 is greater than 95% as determined by SEC-HPLC.
	Human B7-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.