Human B7-H3 (4Ig) /B7-H3b Protein (BH7-HM23B)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human B7-H3 (4Ig), hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 52.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gly27-Thr461

Catalog: BH7-HM23B

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Product Description	Recombinant Human B7-H3 (4Ig)/B7-H3b Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly27-Thr461.
Product Background	B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
Product Category	Recombinant Protein

Target	B7-H3 (4Ig)/B7-H3b
Synonyms	B7-H3; CD276; PSEC0249;-4Ig-B7-H3; UNQ309; PRO352; B7 homolog 3; CD276
Gene/Protein ID	Q5ZPR3-1
Species	Human
Exact Sequence	Gly27-Thr461
Molecular Weight	The protein has a predicted MW of 73.4 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human B7-H3 (4Ig), hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 52.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human B7-H3 (4Ig), hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 52.3ng/ml determined by ELISA.
	The purity of Human B7-H3 (4Ig) is greater than 95% as determined by SEC-HPLC.
	Human B7-H3 (4Ig) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.