Human B7-H3/CD276 Protein (BH7-HM173)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human B7-H3, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu29-Pro245

Catalog: BH7-HM173

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Product Description Recombinant Human B7-H3/CD276 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu29-Pro245.

Product Background

 B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.

Product Category

 Recombinant Protein

Target

 B7-H3/CD276
Synonyms B7H3; B7-H3; CD276; PSEC0249;-UNQ309; PRO352; B7 homolog 3; CD276

Gene/Protein ID

 Q5ZPR3-2

Species

 Human

Exact Sequence

 Leu29-Pro245
Molecular Weight The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Human B7-H3, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human B7-H3, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
Human B7-H3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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