Human BAFFR/TNFRSF13C Protein (BAF-HM10R)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human BAFFR, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-BAFFR Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ser7-Ala71

Catalog: BAF-HM10R

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Product Description	Recombinant Human BAFFR/TNFRSF13C Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser7-Ala71.
Product Background	BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF.
Product Category	Recombinant Protein

Target	BAFFR/TNFRSF13C
Synonyms	CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin
Gene/Protein ID	Q96RJ3-1
Species	Human
Exact Sequence	Ser7-Ala71
Molecular Weight	The protein has a predicted MW of 7.6 kDa. Due to glycosylation, the protein migrates to 13-25 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human BAFFR, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-BAFFR Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF Trimer, hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
	Immobilized Human BAFFR, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-BAFFR Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA.
	Immobilized Human BAFFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF (Trimer) , His Tag with the EC50 of 69.9ng/ml determined by ELISA (QC Test).
	Human BAFFR on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.