Human BDCA-2 Protein (BCA-HM202)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human BDCA-2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Phe46-Ile213

Catalog: BCA-HM202

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Product Description	Recombinant Human BDCA-2 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Phe46-Ile213.
Product Background	BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.
Product Category	Recombinant Protein

Target	BDCA-2
Synonyms	BDCA-2; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; CLEC4C; CD303; BDCA2MGC125791; CD303; CLECSF11PRO34150; DLEC; DLECMGC125792; HECLMGC125793; MGC125789; PRO34150
Gene/Protein ID	Q8WTT0-1
Species	Human
Exact Sequence	Phe46-Ile213
Molecular Weight	The protein has a predicted MW of 47.1 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human BDCA-2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human BDCA-2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA.
	Human BDCA-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.