Human BDCA-2 Protein (BCA-HM302)

Express System: HEK293
Product Tag: N-mFc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human BDCA-2, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Phe46-Ile213

Catalog: BCA-HM302

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Product Description	Recombinant Human BDCA-2 Protein is expressed from HEK293 with mFc tag at the N-Terminus.It contains Phe46-Ile213.
Product Background	BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.
Product Category	Recombinant Protein

Target	BDCA-2
Gene/Protein ID	Q8WTT0-1
Species	Human
Exact Sequence	Phe46-Ile213
Molecular Weight	The protein has a predicted MW of 46.39 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human BDCA-2, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-mFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl, 200mM L-arginine (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human BDCA-2, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.
	Human BDCA-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.