Human Beta Klotho Protein (KLB-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Met30-Thr983

Catalog: KLB-HM101

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human Beta Klotho Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met30-Thr983.
Product Background	Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites.
Product Category	Recombinant Protein

Target	Beta Klotho
Synonyms	betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; MGC142213
Gene/Protein ID	Q86Z14
Species	Human
Exact Sequence	Met30-Thr983
Molecular Weight	The protein has a predicted MW of 111.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).
	Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA (QC Test).
	Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody2, hFc Tag with the EC50 of 34.6ng/ml determined by ELISA (QC Test).
	Immobilized Human FGF21, mFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 1.48ug/ml determined by ELISA.
	The purity of Human Beta Klotho is greater than 95% as determined by SEC-HPLC.
	Human Beta Klotho on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.