Human CA12/Carbonic anhydrase XII Protein (CAS-HM112)
Catalog: CAS-HM112
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| Product Description | Recombinant Human CA12/Carbonic anhydrase XII Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala25-Ser301. |
Product Background |
Carbonic anhydrases (CAs) are a family of enzymes involved in the pH regulation of metabolically active cells/tissues. Carbonic anhydrase XII (CA XII) is a key mediator of several signaling pathways that are involved in cancer development. |
Product Category |
Recombinant Protein |
Target |
CA12/Carbonic anhydrase XII |
Gene/Protein ID |
O43570-1 |
Species |
Human |
Exact Sequence |
Ala25-Ser301 |
| Molecular Weight | The protein has a predicted MW of 32.22 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Measured by its esterase activity. The specific activity is > 40 pmol/min/µg. |
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The purity of Human CA12 is greater than 95% as determined by SEC-HPLC. |
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Human CA12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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